欧美边做饭边被躁bd在线看,欧美激情一区二区三区,亚洲熟妇av一区二区三区宅男,国产av在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1739AGS 人胃腺癌細(xì)胞

AGS 人胃腺癌細(xì)胞

簡(jiǎn)要描述:CRL-1739 AGS 人胃腺癌細(xì)胞
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國(guó)典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來(lái)電咨!

  • 產(chǎn)品型號(hào):CRL-1739
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:2262

產(chǎn)品分類

Product Category

詳細(xì)介紹

RL-1739 AGS 人胃腺癌細(xì)胞 的詳細(xì)介紹

AGS 人胃腺癌細(xì)胞

ATCC® Number:  CRL-1739™

Designations:  AGS

Depositors:   SC Barranco

Biosafety Level: 2

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: stomach

Disease: gastric adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: 1979

Applications: transfection host (Nucleofection technology from Lonza)

Tumorigenic: Yes

Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1;8) (q12;p23), der(19)t(19;?) (q13.6;?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Age:  54 years

Gender:  female


Ethnicity:  Caucasian

Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection .Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  20 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22748: Barranco





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
高清国产av一区二区三区| 久久久久精品免费a片喷水| 99国产精品久久久久久久成人| 国产在线精品一区二区| 欧美成人精品第一区| 性欧美大战久久久久久久| 少妇特黄a一区二区三区| 永久免费av无码网站性色av| 国产chinesehdxxxx宾馆tube| 国产精品乱码在线观看| 亚洲av午夜精品一区二区三区| 与子敌伦刺激对白播放| 特黄把女人弄爽又大又粗| 色一情一乱一伦一区二区三区日本 | 国产又色又爽又刺激在线观看| 色欧美片视频在线观看| 中文人妻熟女乱又乱精品| 精品丝袜人妻久久久久久| 人妻秘书汗と接吻に満ちた视频 | 无遮挡边吃摸边吃奶边做| 好男人视频在线观看免费完整版| 国产精品无码mv在线观看| 精品一二三区久久aaa片| 无码人妻久久一区二区三区69 | 中文在线ずっと好きだった| 欧美精品videoss另类日本| 国产在视频线精品视频| 日产无码久久久久久精品| 欧美性猛交xxxx| 久久人人爽人人爽人人片av| 免费直播在线直播观看| 99国产精品国产精品九九| 高潮毛片无遮挡高清免费视频| 亚洲av无码成人精品区狼人影院| 韩国电影办公室的在线观看| 特黄把女人弄爽又大又粗| 国产剧情av精品迷奷| 爱的色放在线播放| 国产高清不卡一区二区| 亚洲av无码成人精品国产| 亚洲同性男男gv在线观看|